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eDNA_Stream_E01/workflow/Snakefile
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2026-10-04 10:41:13 +01:00

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import os
from dotenv import load_dotenv
load_dotenv()
configfile: "config/main.yaml"
configfile: "config/dependencies.yaml"
configfile: "../reference/species_list.yaml"
module preparation:
snakefile: "rules/preparation.smk"
config: config
module baseline:
snakefile: "rules/baseline_pipeline.smk"
config: config
module ml:
snakefile: 'rules/ml_pipeline.smk'
config: config
# Pulldown
rule all:
input:
#"binaries/datasets",
#f"{config['dorado_model_dir']}/{config['fast_model_name']}",
#f"{config['dorado_model_dir']}/{config['hac_model_name']}",
#'../data/reference_genomes/full_reference.mmi',
#'../data/raw_pod5/'
#'../data/pod5_files_to_pull',
#'../data/basecalled_reads/hac.fastq.gz'
#expand('../data/raw_pod5/PBK98658_853a956f_57f83f46_{batch}.pod5',batch=range(1,config["pod5_dataset_size"]+1,config["pod5_stride"]))
'../data/aligned_reads/nomiss_hac_to_genome.sorted.bam',
'../data/aligned_reads/trap_hac_to_genome.sorted.bam'
#'../data/ml_inputs/hac_label_store.pq'
#'../data/ml_inputs/model_layouts/cnn_512_4_100000_11_4.json'
use rule * from preparation
use rule * from baseline
use rule * from ml